Identification of a small rna containing the trypanosome spliced leader: A donor of shared 5′ sequences of trypanosomatid mRNAs?
Identifieur interne : 001639 ( Main/Exploration ); précédent : 001638; suivant : 001640Identification of a small rna containing the trypanosome spliced leader: A donor of shared 5′ sequences of trypanosomatid mRNAs?
Auteurs : Michael Milhausen [États-Unis] ; Richard G. Nelson [États-Unis] ; Susan Sather [États-Unis] ; Murray Selkirk [États-Unis] ; Nina Agabian [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1984.
English descriptors
- Teeft :
- Agabian, Antigenic variation, Boothroyd, Brucei, Brucei sequence, Clone, Collosoma, Collosoma probe, Cruzi, Cruzi probe, Delange, Experimental procedures, Fasciculata, Gene, Hybridization, Hybridize, Hybridized, Intermolecular, Intron, Leptomonas collosoma, Mrna, Nucl, Nuclease, Nuclease analysis, Nucleotide, Nucleotide oligomer, Polymerase, Precursor, Primer, Probe, Reiteration, Reiteration unit, Reiteration units, Rna, Sense strand, Small rnas, Splice, Splice site, Structural genes, Tandem, Target gene, Target genes, Target mrnas, Transcript, Transcription, Trypanosoma, Trypanosoma brucei, Trypanosomatid, Trypanosomatid mrnas, Trypanosome, Trypanosome mrnas, Unpublished data.
Abstract
Abstract: The 35 nucleotide spliced leader (SL) sequence is found on the 5′ end of numerous trypanosome mRNAs, yet the tandemly organized reiteration units encoding this leader are not detectably linked to any of these structural genes. Here we report the presence of a class of discrete small SL RNA molecules that are derived from the genomic SL reiteration units of Trypanosoma brucei, Trypanosoma cruzi, and Leptomonas collosoma. These small SL RNAs are 135, 105, and 95 nucleotides, respectively, and contain a 5′-terminal SL or SL-like sequence. S1 nuclease analyses demonstrate that these small SL RNAs are transcribed from continuous sequence within the respective SL reiteration units. With the exception of the SL sequence and a concensus donor splice site immediately following it, these small RNAs are not well conserved. We suggest that the small SL RNAs may function as a donor of the SL sequence in an intermolecular process that places the SL at the 5′ terminus of many trypanosomatid mRNAs.
Url:
DOI: 10.1016/0092-8674(84)90267-8
Affiliations:
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Nelson</name></author><author><name sortKey="Sather, Susan" sort="Sather, Susan" uniqKey="Sather S" first="Susan" last="Sather">Susan Sather</name></author><author><name sortKey="Selkirk, Murray" sort="Selkirk, Murray" uniqKey="Selkirk M" first="Murray" last="Selkirk">Murray Selkirk</name></author><author><name sortKey="Agabian, Nina" sort="Agabian, Nina" uniqKey="Agabian N" first="Nina" last="Agabian">Nina Agabian</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9DAE304EC0CD34CD92073AAFE1A14A6CB0C87F01</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0092-8674(84)90267-8</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-PK8ZP5XN-M/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000461</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000461</idno><idno type="wicri:Area/Istex/Curation">000441</idno><idno type="wicri:Area/Istex/Checkpoint">000766</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000766</idno><idno type="wicri:doubleKey">0092-8674:1984:Milhausen M:identification:of:a</idno><idno type="wicri:Area/Main/Merge">001666</idno><idno type="wicri:Area/Main/Curation">001639</idno><idno type="wicri:Area/Main/Exploration">001639</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Identification of a small rna containing the trypanosome spliced leader: A donor of shared 5′ sequences of trypanosomatid mRNAs?</title><author><name sortKey="Milhausen, Michael" sort="Milhausen, Michael" uniqKey="Milhausen M" first="Michael" last="Milhausen">Michael Milhausen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Biochemistry SJ-70 University of Washington Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Nelson, Richard G" sort="Nelson, Richard G" uniqKey="Nelson R" first="Richard G." last="Nelson">Richard G. Nelson</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Biochemistry SJ-70 University of Washington Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Sather, Susan" sort="Sather, Susan" uniqKey="Sather S" first="Susan" last="Sather">Susan Sather</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Biochemistry SJ-70 University of Washington Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Selkirk, Murray" sort="Selkirk, Murray" uniqKey="Selkirk M" first="Murray" last="Selkirk">Murray Selkirk</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Biochemistry SJ-70 University of Washington Seattle</wicri:cityArea></affiliation></author><author><name sortKey="Agabian, Nina" sort="Agabian, Nina" uniqKey="Agabian N" first="Nina" last="Agabian">Nina Agabian</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Washington (État)</region></placeName><wicri:cityArea>Department of Biochemistry SJ-70 University of Washington Seattle</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">38</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="721">721</biblScope><biblScope unit="page" to="729">729</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Agabian</term><term>Antigenic variation</term><term>Boothroyd</term><term>Brucei</term><term>Brucei sequence</term><term>Clone</term><term>Collosoma</term><term>Collosoma probe</term><term>Cruzi</term><term>Cruzi probe</term><term>Delange</term><term>Experimental procedures</term><term>Fasciculata</term><term>Gene</term><term>Hybridization</term><term>Hybridize</term><term>Hybridized</term><term>Intermolecular</term><term>Intron</term><term>Leptomonas collosoma</term><term>Mrna</term><term>Nucl</term><term>Nuclease</term><term>Nuclease analysis</term><term>Nucleotide</term><term>Nucleotide oligomer</term><term>Polymerase</term><term>Precursor</term><term>Primer</term><term>Probe</term><term>Reiteration</term><term>Reiteration unit</term><term>Reiteration units</term><term>Rna</term><term>Sense strand</term><term>Small rnas</term><term>Splice</term><term>Splice site</term><term>Structural genes</term><term>Tandem</term><term>Target gene</term><term>Target genes</term><term>Target mrnas</term><term>Transcript</term><term>Transcription</term><term>Trypanosoma</term><term>Trypanosoma brucei</term><term>Trypanosomatid</term><term>Trypanosomatid mrnas</term><term>Trypanosome</term><term>Trypanosome mrnas</term><term>Unpublished data</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The 35 nucleotide spliced leader (SL) sequence is found on the 5′ end of numerous trypanosome mRNAs, yet the tandemly organized reiteration units encoding this leader are not detectably linked to any of these structural genes. Here we report the presence of a class of discrete small SL RNA molecules that are derived from the genomic SL reiteration units of Trypanosoma brucei, Trypanosoma cruzi, and Leptomonas collosoma. These small SL RNAs are 135, 105, and 95 nucleotides, respectively, and contain a 5′-terminal SL or SL-like sequence. S1 nuclease analyses demonstrate that these small SL RNAs are transcribed from continuous sequence within the respective SL reiteration units. With the exception of the SL sequence and a concensus donor splice site immediately following it, these small RNAs are not well conserved. We suggest that the small SL RNAs may function as a donor of the SL sequence in an intermolecular process that places the SL at the 5′ terminus of many trypanosomatid mRNAs.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Milhausen, Michael" sort="Milhausen, Michael" uniqKey="Milhausen M" first="Michael" last="Milhausen">Michael Milhausen</name></region><name sortKey="Agabian, Nina" sort="Agabian, Nina" uniqKey="Agabian N" first="Nina" last="Agabian">Nina Agabian</name><name sortKey="Nelson, Richard G" sort="Nelson, Richard G" uniqKey="Nelson R" first="Richard G." last="Nelson">Richard G. Nelson</name><name sortKey="Sather, Susan" sort="Sather, Susan" uniqKey="Sather S" first="Susan" last="Sather">Susan Sather</name><name sortKey="Selkirk, Murray" sort="Selkirk, Murray" uniqKey="Selkirk M" first="Murray" last="Selkirk">Murray Selkirk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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